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Home » Immunology » IgG Subclass detection- RID

IgG Subclass detection- RID

Deficiencies of IgG subclasses are an indication of a disturbed immune response.

The major clinical indication for measuring IgG subclasses is the occurrence of abnormally frequent and/or prolonged of severe infections that cannot be explained by usual clinical and laboratory data.

Radioimmunodiffusion (RID) is widely used technique and easy to perform.

The Sanquin kit utilises polyclonal, monospecific antisera in the PeliRIDe™ RID plates to give sharp, single precipitation rings for each IgG subclass.

Diameters of the clearly visible precipitation rings should be measured within 64 hours.

IgG subclass concentrations are determined by means of reference curves or use of a reference table.

RID

IgG1

IgG2

IgG3

IgG4

Intra-assay variation (%)

4

1

1

2

Inter-assay variation (%)

6

2

4

2

Incubation time (mins)

> 64 hours


Product Name    
IgG ANTISERA RID - 4x 1ml CLB-M1175
IgG RID KIT- - 4X (3X8 WELL) CLB-M1557
IgG2 ANTISERA RID - 1ml CLB-M1102
IgG3 ANTISERA RID - 1ml CLB-M1104
IgG4 ANTISERA RID - 1ml CLB-M1106

 

 

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